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Tissue sample pellets were re-suspended with 1N NaOH for protein quantification, using DC (detergent compatible) protein assay, to normalize the concentrations of neurotransmitters to the relative protein contents

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The resulting extract was initially filtered through four layers of gauze, followed by three layers of filter paper, to obtain a clarified filtrate according to an empirical method [8]

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Transcultural adaptation and validation of the CRAFFT as a screening test for problematic alcohol and substance use, abuse and dependence in a group of argentine adolescents