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The pellets were resuspended in RIPA buffer and centrifuged at 14,000 g to acquire nuclear extracts

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We have asserted all along that the that FDA was premature in ending the shortage, because even though the drug came off the shortage list and compounders could no longer prepare it, patients could not get the FDA approved drug, Brunner said

Maintenance phase (weeks 5+): Reduced frequency to 3-5 times per week, with adjusted dosages