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Therefore, it is necessary to strictly control plant care when conducting VIGS experiments ( Age or Development Stages of Infected Plants One study showed that Arabidopsis seedlings inoculated at the two- to three-leaf stage and grown under 16-h light displayed the photo-bleaching phenotype indicative of AtPDS silencing in almost 100% of the cases examined, while older seedlings inoculated at the same conditions exhibited reduced AtPDS transcript levels (95%) in the silenced plants ( N

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