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And similar to X-crystallography, protein NMR studies prioritized the 3D structure determination, mostly neglecting flexible regions, such as linkers, loops, and sequence termini (Marion, 2013)

The diagnosis of type 1 diabetes was according to standard clinical and biochemical parameters (acute onset, ketosis at presentation, insulin therapy from diagnosis and mandatory on-going insulin need, C-peptide levels)

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Reaction was stopped by adding 2N H 2 SO 4 and absorbance was recorded at 450 nm