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For subsequent experiments, 10 nM (first dose to decrease viability), 1 nM (highest dose not affecting viability), and 0.1 nM (dose before 1 nM) were selected

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315 The expression levels of GLP-1R, PKA/CREB signaling pathway components, and inflammation-related proteins (such as TNF-, IL-1, and IL-6) in the rat knee cartilage tissue were measured using Western blot and immunoprecipitation techniques

Ipamorelin binds GHS-R1a, a Gq-coupled receptor, activating phospholipase C to generate IP3 and DAG, mobilising intracellular Ca and amplifying the GH pulse independently of the cAMP arm

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