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The GAPDH substrate 1,3 bisphosphoglycerate was generated by incubating 13.5 mM 3-phosphoglycerate, 6 mM ATP with 0.012 U/l 3-phosphoglycerate phosphokinase in 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 15 mM MgCl 2 for 20 to 30 min at RT

doi: 10.3390/plants12162942 311 YaroshkoO.VasylenkoM.GajdoovA.MorgunB.KhrystanO.VelykozhonL.et al

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A 0.3 mL syringe has a smaller barrel capacity than a 1 mL syringe

However, whether excess hemin exposure from the hemolysis in SCD will affect CBS expression and the generation of GSH in oxidative and ferroptosis stress response has not been investigated