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16,51,52 Herniated discs trigger an innate and adaptive immune response with infiltration of macrophages, T and B cells, and the up-regulation of a myriad of pro-inflammatory cytokines, e.g., IL-1, IL-6, IL-8, IL-10, IL-17, IFN-, CCL2, TNF- and PGE2

200 L tissue homogenate was mixed into a tube pre-added with 800 L of ice-cold methanol/water 80% (vol/vol) followed by vortexing rigorously for 1 min, and then centrifuge at ~ 20,160 g for 15 min in a refrigerated centrifuge

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By dampening inflammatory responses within the CNS, these compounds present a compelling strategy for attenuating disease-associated inflammation and preserving neuronal health