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Cells were seeded on low-attachment culture vessels (Corning) and cultured in mTeSR1 medium supplemented with 40 g ml 1 Matrigel, 1 ng ml 1 BMP4 (Invitrogen) and 10 M Rho kinase inhibitor (ROCK) (R&D) under hypoxic environment with 5% O 2

Image quantification was performed using the color deconvolution plugin in Fiji software 114 to separate the DAB channel and the hematoxylin channel

Protection measures include: Purchase exclusively from state-licensed pharmacies requiring valid prescriptions Avoid online sellers offering GLP-1s without prescription requirements Check lot numbers against FDA alertsavoid products with lot numbers PAR0362, NAR0074, and specific serial numbers Identify warning signs: online pharmacies refusing insurance or restricting pharmacy selection Never use GLP-1 medications prescribed for another patient, as this creates potentially life-threatening complications

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10.1016/j.cell.2021.12.003 Cell 77 WangL.DingJ.ZhuC.GuoB.YangW.HeW.et al (2021)