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Furthermore, the redundancy of pathways in the metabolic rewiring that can take place during isoleucine catabolism to succinyl-CoA is enormous, see Supplementary Fig

For medium composition optimization, molasses (Konem Bio Co., Republic of Korea) and CSL (Shanghai Aladdin Bio-Chem Technology Co., China) were used as carbon and nitrogen sources

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Direct the stream of water down the side of the vial and swirl gently

Nevertheless, the overall contribution of this residual VKR activity to VKD carboxylation in TKO cells appears to be trivial when the relative activity was compared with wild-type HEK293 cells (Supplementary Fig