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TNB undergoes a further ionization in neutral and alkaline pH in water to form the yellow-colored TNB 2 species, the absorbance of which is quantied using a plate reader at 412 nm

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The participants in this study had a mean age of 65 years, mean BMI of 27 kg/m 2 , mean fasting glucose of 9.0 mmol/l and mean HbA1c 7.1% (54 mmol/mol)

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