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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mM butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, Valencia, CA, USA) and sonicated for 30 sec in an ice bath (Ultrasonic bath, Branson, MO, USA)
Sample Weekly Schedule Here is what a typical week looks like on this stack: Monday through Friday: Morning: Topical GHK-Cu applied to face and any target areas after cleansing Any time of day: Injectable GHK-Cu 200 to 600 mcg (if using injectable form) Evening: Optional second topical GHK-Cu application One day per week (your chosen semaglutide day): Semaglutide injection at your usual time (different site from GHK-Cu) Continue GHK-Cu as normal Weekend (if using 5-day injectable schedule): Continue topical GHK-Cu Skip injectable GHK-Cu (some patients prefer a 5 days on, 2 days off schedule for convenience, though daily is also fine) Expanding the Stack Some patients choose to add additional compounds to this foundation
This genetic mapping helps PlexusDx identify your optimal medication type and initial dosing strategy, increasing the likelihood of success with semaglutide or other GLP-1 therapies
Gut Microbes 7(3):189200 Kobyliak N, Conte C, Cammarota G, Haley AP, Styriak I, Gaspar L et al (2016) Probiotics in prevention and treatment of obesity: a critical view
