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It is also very necessary to identify if TMAO contributes to chondrocytes injury through modulating the classical pathways of ferroptosis such as ACSL4/GPX4 or other specific pathways, such as those of iron-related proteins, lipid peroxidation products, and other sensitivities to mechanical stimulation

Subsequently, colonies were fixed with 4% paraformaldehyde for 25 min and stained with 0.2% crystal violet solution for 30 min at room temperature (25-30C)