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In contrast, exogenous leptin, at the dose (10 g/kg ip) that raised plasma leptin to the level occurring postprandially, reduced markedly plasma levels of ghrelin and attenuated food intake and these effects can be reversed by the administration of specific IgG anti-leptin antibodies (28)

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Total RNA was extracted as described above and submitted to Novogene (Sacramento, CA) for quality control, mRNA enrichment, library preparation, and paired-end 150 bp Illumina sequencing