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Glutathione levels decline with age in both blood and brain tissue, a pattern documented across multiple studies, including a 2023 systematic review published in NeuroImage: Clinical

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The fresh tissue was thoroughly homogenised in liquid nitrogen following extraction in ice-cold buffer containing100 mM TrisCl (pH 7.8) buffer containing 10 mM MgCl 2 , 1 mM PMSF, 10 mM DTT, 100 mM EDTA, and 2% PVP, 0.1% protease inhibitor