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Arad G, Freikopf A, Kulka RG

Three 10-min washes with wash solution (0.5% (w/v) skim milk powder, 0.1% Tween20, 1x PBS) were conducted, shaking, at room temperature, followed by a 2 h incubation with a secondary antibody (Rabbit IgG HRP Linked F (ab)2, GENA9340